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human egfr family  (Genentech inc)

 
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    Genentech inc human egfr family
    Human Egfr Family, supplied by Genentech inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+egfr+family/human+egfr+family/pm17303515-39-0-3
    Average 90 stars, based on 1 article reviews
    human egfr family - by Bioz Stars, 2026-10
    90/100 stars

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    Article Title: Targeting metastatic and advanced breast cancer.
    Article Snippet: VER THE past 10 years, breast cancer research has yielded an amazing array of new diagnostic and treatment approaches, but an overall cure for and absolute reventive method against breast cancer continue to elude scienists.. In the United States, more than 40,000 women die from etastatic breast cancer (MBC) on an annual basis.. When breast ancer recurs, the focus shifts from cure to palliation of disease nd disease-related side effects, and maintaining quality of life QOL).



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    Fig. 1. Expression of EGFR family proteins in human cancer cell lines. A. Detection of selected EGFR family proteins (EGFR, HER2, HER3 and HER4) in human cancer cell lines. The cell lines are cultured in vitro to approximately 80% confluence and then lysed and subjected to Western blot analysis of selected EGFR family proteins. Ten µg of the total proteins is loaded in each lane. B. The MCF-7, SKBR-3 and MDA-MB- 231 breast cancer cells are treated with RES at indicated concentrations for 48 h. The cell extracts are prepared and sub ject to Western blot analysis. Ten μg of the total proteins is loaded in each lane except for HER2 detection, in which only 2 μg of the total proteins are loaded. C. The MCF-7, SKBR-3 and MDA-MB-231 breast cancer cells are treated with RES at 10 μM for the indicated length of time. HER3 mRNA level is analyzed by q-PCR. Each data point is mean ± S.D. of three measurements. * P < 0.05, ** P < 0.01 compared to the respective vehicle control.

    Journal: Cancer treatment and research communications

    Article Title: Mechanism underlying resveratrol's attenuation of paclitaxel cytotoxicity in human breast cancer cells: Role of the SIRT1-FOXO1-HER3 signaling pathway.

    doi: 10.1016/j.ctarc.2021.100386

    Figure Lengend Snippet: Fig. 1. Expression of EGFR family proteins in human cancer cell lines. A. Detection of selected EGFR family proteins (EGFR, HER2, HER3 and HER4) in human cancer cell lines. The cell lines are cultured in vitro to approximately 80% confluence and then lysed and subjected to Western blot analysis of selected EGFR family proteins. Ten µg of the total proteins is loaded in each lane. B. The MCF-7, SKBR-3 and MDA-MB- 231 breast cancer cells are treated with RES at indicated concentrations for 48 h. The cell extracts are prepared and sub ject to Western blot analysis. Ten μg of the total proteins is loaded in each lane except for HER2 detection, in which only 2 μg of the total proteins are loaded. C. The MCF-7, SKBR-3 and MDA-MB-231 breast cancer cells are treated with RES at 10 μM for the indicated length of time. HER3 mRNA level is analyzed by q-PCR. Each data point is mean ± S.D. of three measurements. * P < 0.05, ** P < 0.01 compared to the respective vehicle control.

    Article Snippet: For determining the percentage of dead cells, a total 50 μL of trypan blue dye solution (Life Technology) is added to 50 μL of cell suspension, and manual counting of the living (unstained) and dead (stained) cells is performed under a microscope with a hematocytometer. siRNA experiments siRNA duplexes targeting the human EGFR family proteins and scrambled non-targeting siRNAs are obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

    Techniques: Expressing, Cell Culture, In Vitro, Western Blot, Control